Determination of alkylphenols in water samples using liquid chromatography-tandem mass spectrometry after pre-column derivatization with dansyl chloride
| Název česky | Stanovení alkylfenolů ve vzorcích vody pomocí kapalinové chromatografie a tandemové hmotnostní spektrometrie po předkolonové derivatizaci dansyl chloridem |
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| Autoři | |
| Rok publikování | 2015 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Chromatography A |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.sciencedirect.com/science/article/pii/S002196731501314X |
| Doi | http://dx.doi.org/10.1016/j.chroma.2015.09.030 |
| Obor | Analytická chemie, separace |
| Klíčová slova | Alkylphenols; Liquid chromatography; Electrospray ionization; Tandem mass spectrometry; Chemical derivatization of alkylphenols |
| Popis | The present study describes an effect of reaction condition of pre-column derivatization of alkylphenols (APs): bisphenol A (BPA), 4-tert-octylphenol (4-t-OP), 4-octylphenol (4-OP), 4-n-nonylphenol (4-n-NP), and isomers of 4-nonylphenol (iso-NP) with 5-(dimethylamino) naphthalene-1-sulfonyl chloride (dansyl chloride, DNSC) on their LC–ESI–MS/MS determination in water samples. Chemical derivatization improves the sensitivity and selectivity of LC–MS/MS analysis. In principle, alkylphenols can be analyzed by LC–MS/MS without derivatization. However, pre-column derivatization of APs increases the sensitivity up to 1000 times in comparison with the analysis of underivatized alkylphenols. Reaction conditions affecting formation of the DNSC-derivatives, such as various solvent, reaction temperature, reaction time, DNSC concentration and pH values were tested. |
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