Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation.
| Autoři | |
|---|---|
| Rok publikování | 2015 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | he genus Afrodiplozoon was proposed by Khotenovsky in 1981 when Afrodiplozoon polycotyleus (Paperna, 1963) has been exluded from the genus Neodiplozoon Tripathi, 1959 based on the number of clamps on the attachment apparatus. Since then, the inconsistency in the use of the name for this parasite can be seen in the literature. Specimens of A. polycotyleus were collected during a fish survey carried out in April and July 2014 in the Venda region, Limpopo Province, South Africa, from Labeobarbus maraquensis (n=36; mean total length=7.9 cm) and Barbus paludinosus (n=1; total length=6.6 cm). A prevalences of 64% and 59% were recorded for A. polycotyleus during April and July, respectively. Morphological analysis of the composition of the internal organs and attachment clamps using different microscopic methods (light microscopy of stained/unstained specimens and scanning electron microscopy, SEM) provided details for the redescription of the genus. The type material was also studied. Molecular characterization based on the variabily of the second internal transcribed spacer (ITS2) rDNA showed the taxonomic relationship to other representatives of Diplozoidae. Parasites can bear asymetrically from 7 up to 10 clamps in one row on each side of the attachment apparatus, with the first clamp is significantly smaller. The connection sclerite of posterior end of the central plate is wide in all its length. The anterior end of the central plate has short processes. The SEM observations showed the presence of numerous papilae around the mouth of the worm. Molecular characterization and subsequent analysis revealed as a sister species to A. polycotyleus recently described Paradiplozoon bingolensis (Civáňová, Koyun, Koubková, 2013) both positioned far from African representatives of the genus Paradiplozoon (Achmerov, 1974). |
| Související projekty: |