Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm
| Autoři | |
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| Rok publikování | 2016 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Parasitic monogeneans are blood sucking fish ectoparasites. Their presence can lead to significant losses in fish stocks. From a large group of Monogenea (ca 25 000 species) only two species have been deeply sequenced – genomes of Gyrodactylus salaris and Protopolystoma xenopodis and several monogenean protein molecules have been characterized (e.g. cathepsin L – E. nipponicum and Neobenedenia melleni and three unknown serine peptidases from Neobenedenia girellae). In our study of E. nipponicum transcriptome we adopted two different sequencing approaches. 454 pyrosequencing method (GS FLX System, Roche) and sequencing by synthesis method (GS MiSeq, Illumina) to obtain high quality transcriptomic data. We generated 324 941 reads which were assembled into 6910 contigs (454 GS FLX System) and 149 697 864 reads assembled into almost 10 000 unique sequences (Illumina MiSeq) after processing by specific software tools. In this sequences we identified numerous important protein coding transcripts which can be involved in host-parasite interaction and can be essential for life of monogeneans parasites. We determined e. g. cysteine (cathepsins L1, L3, B, C, S and calpain), serine (prolyl endopeptidase, Kunitz-type peptidases and serine protease 33), metallo (nardilysin, paraplegin, XAA-Pro aminopeptidase) and aspartic (cathepsins D and E-A) proteases, their inhibitors (cystatins, salarin, serpins, stefins, smap1 and Kazal-type inhibitors), tegumental antigens and proteins (STARP antigen), potential anticoagulants agents and immunomodulators. The identified E. nipponicum transcripts are similar with those recorded on other worms from phylum Platyhelminthes. |
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