Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

• Autoři: ZOVČÁKOVÁ Monika; ŠPANOVÁ Alena; PANTŮČEK Roman; DOŠKAŘ Jiří; RITTICH Bohuslav
• Rok publikování: 2016
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Analytical Biochemistry
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://www.sciencedirect.com/science/article/pii/S0003269716300884
• Doi: http://dx.doi.org/10.1016/j.ab.2016.05.013
• Obor: Genetika a molekulární biologie
• Klíčová slova: Lanthanide Nd3+ ions; Plasmid DNA; Plasmid size; Pulsed-field agarose gel electrophoresis; Staphylococcus aureus

Přiložené soubory

• 1-s2.0-S0003269716300884-main.pdf

• Popis: Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids—closed circular (ccc), open circular (oc), and linear (lin)—produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.

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