Future of multiplexing
| Autoři | |
|---|---|
| Rok publikování | 2018 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | xMAP technology based on magnetic microspheres represents an open architecture platform, capable of development and adaptation into many multiplex detection assays. Technology is beneficial to end users and therefore it is utilized in various pharmaceutical, clinical and research laboratories not only for nucleic acid assays but also for protein assay formats. On the market, there are available few commercial detection panels, however, these are mainly meant for major clinically important pathogens, infectious diseases or human health. Our research has been focused to fill in the market gaps and develop an in-house assay suitable for detection of genomic DNA of food-borne pathogens (e.g. Trichinella spiralis, Toxoplasma gondii, Giardia intestinalis, Yersinia enterocolitica, Campylobacter jejuni, Salmonella enterica) in various food-related matrices and design the diagnostic system based on the recombinant proteins characteristic for pathogen infections (e.g. Trichinella spp.). Up to this date, we have optimized the working protocol for highly specific multiplex oligonucleotide ligation PCR (MOL-PCR) together with MagPix analysis intended for multiplex detection panel for food-borne pathogens, which is currently still being expanded. The protein assay is under optimization. |
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