Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

• Autoři: KOSTOLANSKÁ Katarína; NOVOTNÁ Lucie; TÁBORSKÁ Eva; PEŠ Ondřej
• Rok publikování: 2019
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Chemical Papers
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: https://link.springer.com/article/10.1007/s11696-018-0560-1
• Doi: http://dx.doi.org/10.1007/s11696-018-0560-1
• Klíčová slova: Endogenous cortisol; Long-term hair concentration; Surrogate analyte LC–MS; Online SPE
• Popis: Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.

Související projekty

• Studium molekulární podstaty vybraných patologických stavů a onemocnění