Formation of Homogenous Interaction Partner Sample for Affinity Studies by Capillary Electrophoresis
| Autoři | |
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| Rok publikování | 2018 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Přiložené soubory | |
| Popis | The capillary electrophoresis-frontal analysis (CE-FA) is method for determination of drug-protein binding parameters in their native states without immobilization or labelling. Classical CE-FA sample preparation process uses off-line mixing reagents outside the capillary. On the other hand, CE can prepares samples directly in the capillary using by electrophoretically mediated microanalysis (EMMA) or transversal diffusion laminar flow profile (TDLFP). Both approaches lead to the other advantages such as smaller sample consumption besides full automation. The main objective of this study was to test the TDLFP methodological approach of sample preparation for CE-FA application. |
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