Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins
| Autoři | |
|---|---|
| Rok publikování | 2019 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | PLOS ONE |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable |
| Doi | http://dx.doi.org/10.1371/journal.pone.0220318 |
| Klíčová slova | lectins; microscopy; agglutination; specificity |
| Popis | Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates. |
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