Omics analyses and functional proteins of Eudiplozoon nipponicum (Monogenea)
| Autoři | |
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| Rok publikování | 2019 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Ectoparasitic flatworms from the group Monogenea represent serious fish pathogens and their presence in the stocks can lead to significant losses in the fish host populations. Despite this fact information related to the biochemical and molecular nature of the physiological processes of these parasites is rather sporadic. Here we introduce novel sequential data set for selected representative E. nipponicum (parasite of common carp) for the purpose to identify functional protein molecules involved in the interaction with the fish host. We started with analyses leading to the generation of E. nipponicum genome, mitochondrial genome, transcriptome and secretome by multiple sequencing techniques (454/Roche, Illumina, Oxford Nanopore) and mass spectrometry analysis, followed by appropriate bioinformatic processing of obtained data. In the E. nipponicum transcriptome (37,062 transcripts) we identified numerous translated proteins potentially involved in the host-parasite interaction (e.g. in the digestion of the host blood), such as e.g. cathepsins B, L1, L3, anticoagulation e.g. Kunitz-type inhibitors, serpins and stefins. Using the mass spectrometry analysis and MEROPS database (BLASTp, E-value 1e-5), 501 E. nipponicum secreted proteins were identified as peptidases and next 107 as inhibitors. Additionally, we made the first bioinformatic steps leading to the second draft of assembly (improved by long Oxford Nanopore reads) and detailed annotation of E. nipponicum genome. According to our data (k-mer counting and flow cytometry), the E. nipponicum genome size was estimated for 1.5 Gb and thus could be one of the biggest within platyhelminth. |
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