Systematic investigation of sequence requirements for DNA i-motif formation

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	SKOLAKOVA P. RENCIUK D. PALACKY J. KRAFČÍK Daniel DVORAKOVA Z. KEJNOVSKA I. BEDNAROVA K. VORLICKOVA M.
Rok publikování	2019
Druh	Článek v odborném periodiku
Časopis / Zdroj	Nucleic acids research
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	https://academic.oup.com/nar/article/47/5/2177/5305265
Doi	http://dx.doi.org/10.1093/nar/gkz046
Klíčová slova	GENE-EXPRESSION; RATIONAL DESIGN; G-QUADRUPLEX; LOOP LENGTH; STABILITY; THERMODYNAMICS; KINETICS
Popis	The formation of intercalated motifs (iMs) secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is reflected by typical changes in CD and UV absorption spectra. By means of spectroscopic methods, electrophoresis, chemical modifications and other procedures, we characterized iM formation and stability in sequences with different cytosine block lengths interrupted by various numbers and types of nucleotides. Particular attention was paid to the formation of iMs at pH conditions close to neutral. We identified the optimal conditions and minimal requirements for iM formation in DNA sequences, and addressed gaps and inaccurate data interpretations in existing studies to specify principles of iM formation and modes of their folding.
Související projekty:	Polymorfní G-quadruplexy v promotorových oblastech genů