Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition
| Autoři | |
|---|---|
| Rok publikování | 2019 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Most posttranscriptional regulation of gene expression is based on RNA elements in mRNAs recognized by RNAbinding proteins (RBPs). Besides primary sequence elements, a second layer of information is embedded in 3’UTRs of mRNAs in the form of RNA structure. Doublestranded RBPs (dsRBPs) can bind structures in 3’UTRs and then exert their function based on dsRNA target recognition through a combination of structure and sequence. Staufen1 (STAU1) is a dsRBP involved in mRNA transport and localization, translational control and mRNA decay by a STAU1mediated mRNA decay (SMD) pathway. The STAU1 binding site (SBS) within human ADPribosylation factor1 (ARF1) 3’UTR is one such target and STAU1 binding to the SBS regulates ARF1 cytoplasmic mRNA levels by the SMD pathway. However, how STAU1 recognizes specific mRNA targets is still unknown. Our structure of the ARF1 SBS STAU1 complex uncovers target recognition by STAU1. STAU1 dsRNA binding domain (dsRBD) 4 interacts with two pyrimidines and one purine from the minor groove side via helix alfa1, beta1beta2 loop anchors the dsRBD at the end of the dsRNA and lysines in helix alfa2 bind to the phosphodiester backbone from the major groove side. STAU1 dsRBD3 displays the same binding mode with specific recognition of one guanine base. Mutants disrupting minor groove recognition of ARF1 SBS reduce SMD in vivo but have minor effect on in vitro binding. Our data suggest how dsRNA recognition by STAU1 mediates diverse functions in gene expression pathways. |
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