Neurodegenerácia a elektrická aktivita: analýza metódou multielectrode array
| Autoři | |
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| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Introduction: Temporal lobe epilepsy is a serious neurological condition associated with neurodegeneration and changes in excitability. Since increased levels of some proteins associated with neurodegeneration, for example the causal proteins of Alzheimer's disease (AD), are associated with an increased risk of epileptic seizures, tissue with the potential to develop AD represents a suitable model for investigating excitatory changes in the development of epilepsy. Methodology: For in vitro analysis of electrical activity and its changes in the presence of neurodegeneration, cerebral organoids differentiated from induced pluripotent cells of a patient with a familial form of AD (nAD = 5) and from a healthy control individual (nWT = 5) were used. Electrical activity was recorded using the multielectrode array technique on differentiation days D72 to D139 (37°C). Subsequently, the analysis of the record took place in 5.-15. min, namely the detection of so-called bursts (groups of spike discharges following each other in a short time interval) and statistical data analysis (median, Mann-Whitney test). Results: An increase in burst activity was detected in some parameters in AD organoids compared to healthy controls. A significant difference was noted in the number of active electrodes (D100, D105, D107, D114, P < 0.05, e.g. D105: WT 1.7 vs. AD 17.0%), average burst duration (D118: WT 153, 3 vs. AD 390.5 ms, P < 0.05), number of bursts (D105: WT 9.5 vs. AD 41.0, P < 0.05) and number of spikes in a burst (D114: WT 7 .0 vs. AD 13.0, P < 0.05). Conclusion: The observed increase in electrical activity points to a possible increase in excitability in neural tissue affected by neurodegeneration. |
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