Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos
| Autoři | |
|---|---|
| Rok publikování | 2024 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Nature Communications |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://www.nature.com/articles/s41467-024-50732-z |
| Doi | http://dx.doi.org/10.1038/s41467-024-50732-z |
| Klíčová slova | zygotic spindle orientation; human embryos; cleavage pattern; nuclear status |
| Popis | The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility. |
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