ELECTROCHEMICAL BIOASSAY FOR DETECTION OF HUMAN PAPILLOMAVIRUS ONCOGENIC ACTIVITY
| Autoři | |
|---|---|
| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Přiložené soubory | |
| Popis | Human papillomavirus (HPV) is known for being the crucial transforming agent of cervical cancer, but also contributes to oropharyngeal and anogenital cancers progression. Cervical cancer is the most common from HPV-induced cancers and belongs among five leading causes of death from tumor diseases in woman worldwide. Higher prevalence is observed in countries lacking preventive programs, comprising especially HPV vaccination and screening. Nowadays, standard screening methods and commercial tests for HPV detection in clinical samples are mostly based on PCR and are designed to detect viral DNA. This approach allows to detect presence of the virus in cells but doesn't provide information about the viral oncogene's transcriptional activity. Therefore, new methods for detection of papillomaviral mRNAs, and cheaper, easier and faster alternatives to PCR at the same time, are being developed. After previous works focused on DNA detection, here we present an EC-LAMP assay for detection of the HPV16 mRNA biomarkers reflecting oncogenic activity of the virus in host cells. In this approach, mRNA levels of three genes were measured: E2 regulation gene, E7 oncogene and 18S rRNA subunit as a housekeeping standard. Ratios between E2 and E7 mRNA levels were calculated, and results compared to clinical data as well as to standard methods, PCR and ddPCR. The assay showed good concordance with both clinical data and standard validation methods. |
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