CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications
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| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
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| Popis | The increasing prevalence of antimicrobial-resistant bacterial pathogens, including multidrug-resistant strains of Staphylococcus aureus, has intensified the need for alternative therapeutic approaches. Particular attention is being paid to phage therapy, which utilizes viral particles infecting bacteria as a key component in the treatment process. Significant advantages of bacteriophages as lytic agents include high specificity (which reduces the impact on natural microflora), their ability to penetrate biofilms, and the potential for phage property modification. The CRISPR-Cas10 technology, a powerful tool for precise genetic engineering, enables targeted modifications of viral genomes by facilitating the selection of recombinant bacteriophages.1 This method holds potential for constructing viral particles with enhanced therapeutic properties, such as an expanded host range or improved lytic efficiency. The novel, unique characteristics also broaden the scope for their application in biotechnology. The use of modified bacteriophages in combination with biosensing technologies opens up new possibilities for applications in diagnostics and environmental monitoring, as well as in research and development. In our study, we focus on modifying the structural proteins of S. aureus therapeutic phages by adding a polyhistidine (His) tag to facilitate the oriented binding of particles to a biosensor chip. To monitor the binding kinetics and confirm particle attachment, we will use Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) biosensors. |
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