Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

TURANEK Jaroslav TURANEK KNOTIGOVA Pavlina KULICH Pavel SKOUPY Radim HRUBANOVA Kamila VAŠKOVICOVÁ Naděžda FEKETE Ladislav KANA Antonin MIKULÍK Robert RASKA Milan

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj Pharmaceuticals
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/1424-8247/17/11/1479
Doi http://dx.doi.org/10.3390/ph17111479
Klíčová slova gold nanoparticles; in vivo imaging; microcomputer tomography; biodistribution; nanotoxicology
Popis The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for mu CT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution mu CT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by mu CT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info