Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	BOUCHAL Pavel GLATZ Zdeněk JANICZEK Oldřich MANDL Martin
Rok publikování	2001
Druh	Článek v odborném periodiku
Časopis / Zdroj	Folia Microbiologica
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Obor	Biochemie
Klíčová slova	rhodanese; CZE
Popis	The new capillary zone electrophoretic method was applied for the assay of the enzymatic activity of the rhodanese from Acidithiobacillus ferrooxidans. The enzyme activities determined by capillary zone electrophoresis were compared with those determined by discontinuous spectrophotometric method. The values obtained were in a good agreement. The method was also used to evaluate Michaelis constants for both substrates; a new approach was developed to solve the problem with variable ionic strength of the samples. The other properties of the enzyme such as the pH and temperature optima were determined as well.
Související projekty:	Acidifikační procesy v sulfidových odpadech Strukturně-funkční vztahy biomolekul a jejich role v metabolismu