Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization
| Autoři | |
|---|---|
| Rok publikování | 2002 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Analytical and Bioanalytical Chemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization |
| Popis | The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181. |
| Související projekty: |