An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve
| Název česky | Imunohistochemická lokalizace SDF-1 a jeho receptoru CXCR4 ve spinálních gangliích |
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| Autoři | |
| Rok publikování | 2007 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Biologická aktivita SDF1 (stromal cell-derived factor) je zprostředkovaná chemokinovým receptorem CXCR4. Imunohistochemická lokaůizace SDF-1 a CXCR4 proteinů byla sledována v C7-C8 a L4-L5 spinálních gangliích intaktních a operovaných potkanů pro unilaterální ligaturu L4-L5 spinálního nervu SNL) a sedacího nervu (ScNL). The naive DRG displayed immunofluorescence for SDF1-IF and CXCR4-IF at the surface of neurons and satellite glial cells (SGC). A significant decrease of SDF1-IF was induced in the ipsilateral, but remained distinct in the contralateral L4-L5 DRG one week after SNL. A bilateral reduction of SDF1-IF was found 2 weeks from SNL. A gradual diminution of SDF1-IF was observed bilaterally in the L4-L5 DRG neurons, but modest IF was in SGC after 1 and 2 weeks from ScNL, respectively. The SNL for 1 week induced a reduction of CXCR4-IF at the SGC surfaces of ipsi- in contrast to contralateral L4-L5 DRG, while intraneuronal staining was increased. Two weeks after SNL, the differences of CXCR4-IF at the neuronal surfaces in ipsilateral L4-L5 DRG and at the SGC surfaces in contralateral ones were marked. CXCR4-IF at the SGC surfaces remained in both ipsi- and contralateral DRG up to 2 weeks from ScNL, but it was increased intraneuronally only in the ipsilateral DRG. Similar pattern of SDF1- and CXCR4-IF was induced in the cervical DRG. A different expression of SDF1-IF and its receptor was observed in ipsi- and contralateral DRG associated and non-associated with unilateral SNL and ScNL. This suggests their different regulation with the time of survival and type of nerve injury. |
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