Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
| Název česky | Vztah struktutry a funkce a inženýrství haloalkán dehalogenáz. |
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| Autoři | |
| Rok publikování | 2008 |
| Druh | Kapitola v knize |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The structure–function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (1) activity, (2) thermostability, (3) substrate specificity and (4) enantioselectivity. Some variants carried mutations in the tunnels, connecting the buried active site with surrounding solvent, rather in the active site itself. Mutagenesis in the residues lining the protein tunnels represents a new paradigm in protein engineering. |
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