Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic.

• Autoři: MATĚJKOVÁ POSPÍŠILOVÁ Petra; FLASAROVÁ Magdalena; ZÁKOUCKÁ Hana; BOŘEK M.; KŘEMENOVÁ Gabriela; ARENBERGER P.; WOZNICOVÁ Vladana; WEINSTOCK G. M.; ŠMAJS David
• Rok publikování: 2009
• Druh: Článek ve sborníku
• Konference: Clinical Microbiology and Infection, Abstracts of the 19th ECCMID, P698
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: http://www3.interscience.wiley.com/cgi-bin/fulltext/122401282/PDFSTART
• Obor: Mikrobiologie, virologie
• Klíčová slova: macrolide resistance; 23S rRNA; syphilis
• Popis: Due to alergies to penicillin, macrolide regimens are frequently applied in the treatment of syphilis. A2058G mutation in the 23S rDNA locus was described to cause erythromycin resistance in syphilis.Molecular detection of syphilis represents the only available test to reveal macrolide resistance in clinical isolates, because T. pallidum can not be cultured in vitro. The set of 28 clinical isolates with detectable genetic material were collected from 22 patients in the Czech Republic in the time period 2005 to 2008. Four patients (18.2%) were diagnosed with a strain bearing the A2058G transition and four patients (18.2%) with a strain bearing the A2059G mutation. Screening of clinical samples revealed a novel mutation at the position 2059 of the T. pallidum 23S rRNA gene that causes macrolide resistance in several other bacteria. Our results show that macrolide resistant isolates of T. pallidum bearing A2058G and A2059G mutations are relatively abundant in the Czech Republic.

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