Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis
| Autoři | |
|---|---|
| Rok publikování | 2010 |
| Druh | Článek ve sborníku |
| Konference | Acta Universitatis Palackianae Olomucensis Chemica 47S |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | oligonucleotides osmium tetroxide capillary electrophoresis |
| Popis | Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied. Capillary zone electrophoresis as an analytical technique was chosen for the sufficient ability to monitor the changes of the modified oligonucleotides. |
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