An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry
| Autoři | |
|---|---|
| Rok publikování | 2010 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Short oligonucleotides play an important role in many biological processes including triplet repeat expansion associated with neurodegenerative diseases (fragile X syndrome, Huntington’s disease, Friedreich’s ataxia or myoclonic epilepsy) [1]. One of the most stable DNA fragments, which forms hairpin, is heptamer d(GCGAAGC) [2]. In our electrochemical study we analyzed not only this hairpin but also DNA and RNA fragments containing self-complementary nucleobases (GC) forming the stem. Both DNA and RNA analogues adsorbed on mercury electrodes provided voltammetric reduction signals of adenine and cytosine (A and C) and oxidation signals of guanine (G). We found significant differences between DNA and RNA fragments depending on their length, sequence, and pH (Fig. 1). For the resolution of A and C reduction signals and evaluation of the nature of anodic processes of the G signal in both DNA and RNA fragments, elimination voltammetry with linear scan (EVLS) was utilized [3,4]. |
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