Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques
| Autoři | |
|---|---|
| Rok publikování | 2011 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Background: Although breast cancer is the most common women cancer in the world, traditional prognostic factors are not sufficient for precise risk-group discrimination. This is well illustrated by the group of low grade breast tumors in which small percentage of tumors form lymph node metastases early. Methods: Our effort has been focused on biomarker discovery in the set of 96 breast cancer primary tumors grouped and randomized according to grade, lymph node and hormonal receptor status. iTRAQ-2DLC-MS/MS and 2-D SDSPAGE proteomics approaches were involved in complex protein analysis of tissue lysates. qRT-PCR assays and immunohistochemistry were used as complementary tools for verification of selected gene products expression. Results: Our results indicate several types of differences between the studied groups of tumors, namely: (i) iTRAQ- 2DLC-MS/MS data were internally validated via ErbB2 protein expression as confirmed by immunohistochemistry. (ii) Biological replicates in the study exhibited positive correlation and clustering of proteomic profiles according to clinicopathological parameters. (iii) Expression of selected cytoskeletal proteins correlated with lymph node status of the tumors. (iv) Expression of AGR family members was strongly affected by estrogene receptor status of the tumors. Conclusion: Our findings provide a solid data for targeted functional studies and validation geared towards more indepth description of metastatic mechanisms in low-grade breast cancer tumors. |
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