Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram

• Název česky: Účinky napěťově citlivého barviva di-4-ANEPPS u modelu izolovaného srdce potkana
• Autoři: FIALOVÁ Kateřina; KOLÁŘOVÁ Jana; JANOUŠEK Oto; RONZHINA Marina; PROVAZNÍK Ivo; NOVÁKOVÁ Marie
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Computing in Cardiology
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: http://cinc2011.org
• Obor: Kardiovaskulární nemoci včetně kardiochirurgie
• Klíčová slova: voltage-sensitive dye;isolated heart; rat;electrogram
• Popis: Although the classical suction microelectrodes are still considered golden standard for recording of monophasic action potentials, there are various new methods enabling to record electrical changes from small area on the heart surface. One of them is recording of cardiac electrical activity using voltage-sensitive dyes (VSDs) by optical method. VSDs exhibit large fluorescence and/or absorption changes that vary with changes of the membrane potential. Staining of the heart with the dye should not result in pharmacological or toxic effects to the preparation. Data about response of the heart to staining with VSD are scarce. In order to determine this response in classical biomodel in basic cardiology, we followed arrythmia-preceding parameters in isolated rat heart electrogram during staining with VSD di-4-ANEPPS and washout periods.

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