Bacterial DNA isolation using new magnetic polymer microspheres containing carboxyl groups

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Authors	RITTICH Bohuslav ŠPANOVÁ Alena HORÁK Daniel
Year of publication	2005
Type	Article in Proceedings
Conference	8th Symposium on Lactic Acid Bacteria
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Lactic acid bacteria; DNA; magnetic microspheres; NIPAM
Description	In the study, bacterial DNA of the Bifidobacterium and Lactobacillus genera was isolated from complex dairy products(butter milk, cheese, yoghurt, probiotic tablets) or from cell lyophilisates by magnetic and/or nonmagnetic carriers containing carboxyl groups on the surface. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. Adsorption of bacterial DNA isolated from the above mentioned bacteria on thermosensitive poly(N-isopropylacrylamide, NIPAM) based microspheres was also studied at different temperatures.
Related projects:	Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering Aplication of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality