Komparativní genomika patogenních kmenů T. pallidum.
| Title in English | Comparative genomics of pathogenic strains of T. pallidum. |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | X. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Comparative genomics; pathogenic strains of T. pallidum. |
| Description | The genomes of analysed strains were divided into 97 overlapping intervals covering the whole genome and each of these segments was amplified and digested by restriction enzymes BamH I, EcoR I and Hind III. The resulting restiction profiles were used for identification of heterologous regions in chromosomal DNA of analysed strains. Differences in intergenic region TP0135-6 and differences in number of tandem repetitions within the gene TP470 and genes TP0433-4 (hypothetical genes) were detected in the strains SS14, Samoan D and Cuniculi A when compared to the Nichols genome. Insertion between genes TP0126-7 showed sequence similarity to the tprK gene in all analysed strains. 6 deletions and 1 insertion were found in the strain Samoan D. Deletions were mapped to hypothetical genes TP0067, TP0132-3, TP0136, TP0316 (tprF), TP0317 (tprG) and TP1030-1 (tprL). Insertion of sequentially unique region was detected between genes TP0548-9. 17 deletions were found in the strain Cuniculi A: 4 genes were completely deleted (TP0127, TP0128, TP0134 and TP0619) and 13 partially (TP0104, TP0129, TP0133, TP0135, TP0136, TP0315, TP0316 (tprF), TP0317 (tprG), TP0470, TP0545, TP0548, TP0620 (tprI) and TP1029). With exception of genes TP0104 (ushA, 5-nucleotidase) and TP0545 (methylgalactoside-ABC-transporter) all the above mentioned genes are hypothetical genes. 5 insertions were detected in the strain Cuniculi A: 2 within the genes TP0548 and TP1030 (hypothetical genes) and 3 in intergenic regions TP0548-9, TP0009-10 (part of the sequence of tprA/B gene inserted) and TP0316-7 (sequence similar to the tprI gene inserted). |
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