Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.
| Title in English | Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D |
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| Authors | |
| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopically and serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols (TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain. |
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