Detekce Treponema pallidum subsp. pallidum v biologickém materiálu, výhledy pro epidemiologii syfilis
| Title in English | Detection of Treponema pallidum subsp. pallidum in clinical material, epidemiologic consequences |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | XXI. Pečenkovy epidemiologické dny. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum subsp. pallidum; detection in the clinical material; molecular typing; mucocutaneous swabs |
| Description | The PCR amplification protocol detecting tmpC gene (TP0319, encoding putative membrane lipoprotein) was used for detection of Treponema pallidum subsp. pallidum in the clinical material. T. p. pallidum DNA was not detected in any of the analyzed sera samples. Out of 11 mucocutaneous swabs (7 genital and 4 pharyngeal ones), 6 from 3 patients with primary or secondary syphilis were positive. In addition, one skin swab was positive in patient with early congenital syphilis. Sera and cerebrospinal fluid samples from this patient were also positive. DNA sequencing of genes TP0136 and TP0548 in the positive samples revealed two patients with DNA sequence identical to T. p. pallidum strain SS14 and two unique, not yet known T. p. pallidum strains. The advances in molecular typing of T. p. pallidum in clinical material will become important for epidemiologic consequences and for clinical discrimination between reinfection and reactivation of the syphilitic process. |
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