Molecular dissection of Rad52 mediator activity

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Authors	KREJČÍ Lumír
Year of publication	2007
Type	R&D Presentation
MU Faculty or unit	Faculty of Science
Citation
Description	DNA strand break repair by HR is mediated by genes of the RAD52 epistasis group. Rad52 protein plays central role in this process. It binds and anneals complementary ssDNA, self-associate to form multimeric rings, interacts with Rad51, and promotes nucleation of Rad51 onto ssDNA. Here, we show that Rad52 competes with Srs2 anti-recombinase in mediating Rad51-catalyzed strand exchange reaction through common interaction site on Rad51. The molecular mechanism of Rad52 function in homologous recombination together with the role of postranslational modification (SUMO-ylation) is discussed.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Biomolecular centre Srs2 protein and its multi-functional role in rekombination/repair processes