Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.cssm.info/priloha/fm2007_325.pdf |
| Field | Biochemistry |
| Keywords | Piezoelectric sensor; Quartz crystal microbalance; Immunoassay |
| Description | A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor included mouse polyclonal antibody immobilized through the layer of protein A covalently linked to the gold electrode of the sensor. Thus obtained immunosensor was able to detect F. tularensis with the limit of detection 105 CFU/mL with a typical measuring cycle under 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment. |
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