Optimalizace reversibilní adsorpce vysokomolekulární DNA na magnetické nosiče

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Title in English	Optimization of reversible adsorption of high-molecular DNA on magnetic microspheres
Authors	ŠÁLEK Petr TRACHTOVÁ Štěpánka ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2008
Type	Article in Proceedings
Conference	XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	DNA; reversible adsorption; magnetic microspheres; P(HEMA-co-GMA); carboxyl coated
Description	Genomic DNA were isolated from crude cell lysates of Lactobacillus paracasei subsp. paracasei CCDM 211/06 cells with help of reversible imobilisation on hard carriers. Magnetic nonporous micropheres poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA)covered by carboxyl groups were used for DNA isolation. DNA adsorption on carriers occured in the presence of high NaCl and PEG 6000 concentrations. Quality and amount of isolated DNA were estimated using agarose gel electrophoresis, spectrophotometry and amplification in PCR.
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