CHARACTERISATION OF A NEW LECTIN BCLC FROM THE HUMAN OPPORTUNISTIC PATHOGEN BURKHOLDERIA CENOCEPACIA

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Authors	ŠULÁK Ondřej DELIA Monia KOSTLÁNOVÁ Nikola IMBERTY Anne WIMMEROVÁ Michaela
Year of publication	2008
Type	Article in Proceedings
Conference	XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Lectin; Burkholderia cenocepacia; SPR; ITC
Description	Four genes coding proteins homologous to lectin PA-IIL from Pseudomonas aeruginasa have been found in the genome of B. cenocepacia. One of them, named BclC, is a 28 kDa protein which is able to recognize D-mannosylated carbohydrates. This protein also exhibits partial sequence homology with the protein CV-IIL from the Chromobacterium violaceum. BclC has been cloned and prepared in recombinant form. Sequence analysis showed two distinct domains in the protein, N-terminal part that does not have any sequence homolog in genomic and protein databases, and C-terminal part that codes for lectin domain. Both domains were also cloned separately to simplify further structure-function characterization. Detailed functional studies using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) allowed to define binding properties (afinity, kinetics) and thermodynamic parameters.
Related projects:	Structure-function studies on lectins and adhesins from microbial patogens Biomolecular centre