Optimalizace exprese a purifikace rekombinantního lektinu CV-IIL z patogenního mikroorganismu Chromobacterium violaceum

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.

Title in English	Optimization of expression and purification of recombinant lectin CV-IIL from pathogen Chromobacterium violaceum
Authors	DEJMKOVÁ Eva POKORNÁ Martina WIMMEROVÁ Michaela
Year of publication	2008
Type	Article in Proceedings
Conference	Študentská vedecká konferencia 23.apríl 2008, Zborník prispevkov, 1.zväzok, biologická sekcia
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	lectin; CV-IIL; Chromobacterium violaceum
Description	The protein engineering of the lectin CV-IIL from human opportunistic bacterium Chromobacterium violaceum - site directed mutagenesis. An optimization of CV-IIL ins HisTag protein expresion in E.coli different expresion systems and optimization purification with usage a metalloaffinity chromatography.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Biomolecular centre