Metagenomic and proteomic analysis of dental calculus of abbot Gregor Johann Mendel

• Autoři: CHOCHOLOVÁ Eva; DROZDOVÁ Eva; FIALOVÁ Dana; ROUDNICKÝ Pavel; ZDRÁHAL Zbyněk; ZWINSOVÁ Barbora; NOVOTNÁ Kateřina; PARDY Filip; HYNŠT Jakub; PEŠKA Marek; ZŮBEK Antonín; MRÁZOVÁ Kateřina; KRZYŽÁNEK Vladislav; DOUBEK Michael; POSPÍŠILOVÁ Šárka
• Rok publikování: 2022
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Folia Mendeliana : supplementum ad Acta Musei Moraviae
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://www.mzm.cz/e-shop?ai%5Baction%5D=list&ai%5Bcontroller%5D=Catalog&ai%5Bf_catid%5D=23&ai%5Bf_name%5D=Folia-Mendeliana
• Klíčová slova: Gregor Johann Mendel; oral microbiome; dental calculus; palaeoproteomics; palaeogenomics; periodontitis
• Popis: As part of the interdisciplinary research of abbot and founder of genetics, Gregor Johann Mendel, his dental calculus was sampled to gain insight into his health that could not be obtained by mere genome sequencing. A total of 469 proteins were found after filtering, including proteins related to immunity and oral health. Multiple oral species connected to dental pathology such as periodontitis were confirmed based on both DNA and proteins, originating from Porphyromonas gingivalis, Tannerella forsythia, or Treponema denticola. High abundance of Clostridiales DNA was confirmed, in agreement with mummification by saponification.

Související projekty

• CERIT Scientific Cloud
• Czech Infrastructure for Integrative Structural Biology
• e-Infrastruktura CZ
• Národní centrum lékařské genomiky
• Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10