Synergy testing of biofilm forming Enterobacteriaceae
| Název česky | Testování synergismu u enterobakterií tvořících biofilm |
|---|---|
| Autoři | |
| Rok publikování | 2011 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Six wilde-type strains of Enterobacteriaceae (2 E. coli, 2 K. pneumoniae, 2 E. aerogenes) able to form a biofilm were used. After biofilm detection with modified Christensen method, 24-hours old biofilm in trypticase soy broth medium on 96-well polystyren microtiter plates was prepared. Eight antibiotics, namely cefotaxim, ceftazidim, cefoperazon, cefepim, netilmicin, amikacin, tobramycin, ciprofloxacin, individually and in combinations (netilmicin + ciprofloxacin, cefoperazon + ciprofloxacin, cefoperazon + netilmicin, cefepim + tobramycin, cefotaxim + amikacin, cefotaxim + tobramycin, cefotaxim + netilmicin, ceftazidim + amikacin, ceftazidim + netilmicin, cefepim + amikacin) were tested. Minimum biofilm inhibitory concentration (MBIC) and fractionate inhibitory concentration (FBIC) were determined. The combinations were classified as synergistic, partially synergistic, indifferent, or antagonistic. |
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