Informace o projektu
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Kód projektu
- LM2015043
- Období řešení
- 1/2016 - 12/2019
- Investor / Programový rámec / typ projektu
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Ministerstvo školství, mládeže a tělovýchovy ČR
- Velké infrastruktury pro výzkum, vývoj a inovace
- Fakulta / Pracoviště MU
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Středoevropský technologický institut
- prof. RNDr. Vladimír Sklenář, DrSc.
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec, Prague-West) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies (total investments will exceed 750 mil. CZK by the end of 2015), will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB as National Affiliated Center is a part of the ESFRI projects INSTRUCT.
Publikace
Počet publikací: 252
2017
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Single Molecule Upconversion-Linked Immunosorbent Assay with Extended Dynamic Range for the Sensitive Detection of Diagnostic Biomarkers
Analytical Chemistry, rok: 2017, ročník: 89, vydání: 21, DOI
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Solution structure of domain 1.1 of the sigma(A) factor from Bacillus subtilis is preformed for binding to the RNA polymerase core
Journal of Biological Chemistry, rok: 2017, ročník: 292, vydání: 28, DOI
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Staphylococcus sciuri bacteriophages double-convert for staphylokinase and phospholipase, mediate interspecies plasmid transduction, and package mecA gene
Scientific Reports, rok: 2017, ročník: 7, vydání: April, DOI
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Structural Basis for Polyproline-Mediated Ribosome Stalling and Rescue by the Translation Elongation Factor EF-P
Molecular Cell, rok: 2017, ročník: 68, vydání: 3, DOI
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Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function
Biophysical Journal, rok: 2017, ročník: 112, vydání: 7, DOI
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Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p
EMBO reports, rok: 2017, ročník: 18, vydání: 6, DOI
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Structure and dynamics of the RNAPII CTDsome with Rtt103
Proceeding of the National Academy of Sciences of the USA, rok: 2017, ročník: 114, vydání: 42, DOI
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Structure of a Stable G-Hairpin
Journal of the American Chemical Society, rok: 2017, ročník: 139, vydání: 10, DOI
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Student training (FEEC BUT)
Rok: 2017, druh: Uspořádání workshopu
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Student training (FEEC BUT) II
Rok: 2017, druh: Uspořádání workshopu